Catalog No: OKBB00655
Size:96 Wells
Price: $583.00
SKU
OKBB00655
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||||||||||
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Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva's mouse IL1RL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL1RL1 has been precoated onto 96-well plates. Standards(NS0, S27-R332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL1RL1 amount of sample captured in plate. | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Range | 156 pg/ml - 10,000 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
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ELISA Kit Component |
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Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | ||||||||||||||||||||||||||||||||||||||||||
Sample Type | Cell culture supernatants, serum and plasma (heparin, EDTA) | ||||||||||||||||||||||||||||||||||||||||||
Sensitivity | <10 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
Specificity | Natural and recombinant mouse IL1RL1 | ||||||||||||||||||||||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay | ||||||||||||||||||||||||||||||||||||||||||
Immunogen | Expression system for standard: NS0; Immunogen sequence: S27-R332 | ||||||||||||||||||||||||||||||||||||||||||
Description | For quantitative detection of mouse IL1RL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Gene Symbol | IL1RL1 |
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Gene Full Name | interleukin 1 receptor-like 1 |
Alias Symbols | DER-4, DER4, FIT 1, Growth stimulation expressed, homolog of mouse growth stimulation-expressed, Il1rl1, IL33R, ILRL1_HUMAN, Interleukin 1 receptor like 1, interleukin 1 receptor related protein, Interleukin-1 receptor-like 1, Ly84, Lymphocyte antigen 84, Protein ST2, ST2, ST2L, ST2V, Ste2, Suppressor of tumorigenicity 2, T1, T1 protein |
NCBI Gene Id | 17082 |
Protein Name | Interleukin-1 receptor-like 1 |
Description of Target | Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. |
Uniprot ID | P14719 |
Protein Accession # | NP_001020773.1 |
Nucleotide Accession # | NM_001025602.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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