IL1R2 ELISA Kit (Mouse) (OKCD02631)

Catalog No: OKCD02631

Size:96WELLS

Mouse IL1R2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-102%	84-96%	93-105%	92-99%
  EDTA Plasma (n=5)	80-98%	87-95%	99-105%	84-103%
  Heparin Plasma (n=5)	79-94%	81-88%	90-101%	82-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Type II (IL1R2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Type II (IL1R2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Type II (IL1R2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Receptor Type II (IL1R2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-104	88
  EDTA Plasma (n=5)	81-98	86
  Heparin Plasma(n=5)	82-103	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Type II (IL1R2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Type II (IL1R2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL1R2 Microplate: 96 Wells (12 x 8 Well strips)
  • IL1R2 Lyophilized Standard: 2
  • 100X Biotinylated IL1R2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 5.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Type II (IL1R2). No significant cross-reactivity or interference between Interleukin 1 Receptor Type II (IL1R2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Il1r2
• Gene Full Name: interleukin 1 receptor, type II
• Alias Symbols: CD121 antigen-like family member B;CD121b;Il1;IL-1 receptor beta chain;IL-1 type II receptor;Il1r-2;IL-1R-2;IL-1R-beta;IL-1RT2;IL-1RT-2;interleukin-1 receptor beta;interleukin-1 receptor type 2;Interleukin-1 receptor type II.
• NCBI Gene Id: 16178
• Protein Name: Interleukin-1 receptor type 2
• Description of Target: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (By similarity).
• Uniprot ID: P27931
• Protein Accession #: NP_034685.1
• Nucleotide Accession #: NM_010555.4
• Protein Size (# AA): 410