IL1A ELISA Kit (Human) (OKBB00173)

Catalog No: OKBB00173

Size:96 Tests

Human IL1A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S113-A271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-1 alpha amount of sample captured in plate.
• ELISA Kit Range: 4.7 pg/ml - 300 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	54	147	221	48	139	234
  St. Dev.	1.84	7.497	9.28	2.64	10.29	14.98
  %CV	3.40	5.1	4.19	5.5	7.40	6.40

• ELISA Kit Component:
  • Lyophilized recombinant human IL-1alpha standard: 10 ng/tube x 2
  • Anti-human IL-1alpha Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human IL-1alpha antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 4.7pg/ml-300pg/ml
  Principle: Aviva's human IL-1α ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1α has been precoated onto 96-well plates. Standards
  (E.coli,S113-A271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1α is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1α amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don't let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don't reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant human IL-1α with the molecular mass of 18kDa.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <0.5 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Lafage, M.; Maroc, N.; Dubreuil, P.; de Waal Malefijt, R.; Pebusque, M.-J.; Carcassonne, Y.; Mannoni, P. The human
  interleukin-1-alpha gene is located on the long arm of chromosome 2 at band q13. Blood 73: 104-107, 1989.
  2. Bailly, S.; di Giovine, F. S.; Blakemore, A. I. F.; Duff, G. W. Genetic polymorphism of human interleukin-1-alpha.
  Europ. J. Immun. 23: 1240-1245, 1993.
  3. Modi, W. S.; Masuda, A.; Yamada, M.; Oppenheim, J. J.; Matsushima, K.; O'Brien, S. J. Chromosomal localization of the human interleukin 1 alpha (IL-1-alpha) gene. Genomics 2: 310-314, 1988.
  4. Diehl, S. R.; Wang, Y.; Brooks, C. N.; Burmeister, J. A.; Califano, J. V.; Wang, S.; Schenkein, H. A. Linkage
  disequilibrium of interleukin-1 genetic polymorphisms with early-onset periodontitis. J. Periodont. 70: 418-430, 1999.
  5. Silver, A. R. J.; Masson, W. K.; George, A. M.; Adam, J.; Cox, R. The Il-1 alpha and beta genes are closely linked
  (less than 70 kb) on mouse chromosome 2. Somat. Cell Molec. Genet. 16: 549-556, 1990.
• Specificity: Natural and recombinant human IL-1 alpha
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: S113-A271
• Description: For quantitative detection of human IL-1 alpha in cell culture supernatants, serum and plasma( heparin, EDTA, citrate).

Target Info

• Gene Symbol: IL1A
• Gene Full Name: interleukin 1, alpha
• Alias Symbols: Hematopoietin 1, IL 1 alpha, Il 1a, IL1, IL1 ALPHA, IL1A, IL1F1, Ilia, Interleukin 1 alpha, Interleukin 1 alpha precursor, Interleukin1 alpha, OTTHUMP00000203692, OTTMUSP00000016430, Preinterleukin 1, Pro interleukin 1, Pro interleukin 1 alpha, Prointerleukin 1, RP23-160G19.8
• NCBI Gene Id: 3552
• Protein Name: Interleukin-1 alpha
• Description of Target: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant human IL-1α with the molecular mass of 18kDa.
• Uniprot ID: P01583
• Protein Accession #: NP_000566.3
• Nucleotide Accession #: NM_000575.4