Catalog No: OKEH04132
Size:96T
Price: $651.00
SKU
OKEH04132
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL18 ELISA Kit (Dog) (OKEH04132) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL18 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL18 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL18 captured in the well.
ELISA Kit Range7.8-500pg/mL
ELISA Kit Component
ComponentAmount
Anti-IL18 Microplate96 Wells (12 x 8 Well Strips)
IL18 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
IL18 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity2.4 pg/mL
SpecificityNatural and recombinant Dog Interleukin-18
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL18
Gene Full Nameinterleukin 18
Alias SymbolsIFN-gamma-inducing factor;IGIF;IL-1 gamma;IL-18;interferon gamma-inducing factor;interleukin 18 (interferon-gamma-inducing factor);interleukin-1 gamma;interleukin-18.
NCBI Gene Id403796
Protein NameQ9XSR0
Description of TargetInterleukin-18
Uniprot IDhttps://www.uniprot.org/uniprot/Q9XSR0
Protein Accession #NP_001003169.1
Nucleotide Accession #NM_001003169.1
Protein Size (# AA)193
Molecular Weight22 kDa
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