IL15 ELISA Kit (Dog) (OKCA01045)

Catalog No: OKCA01045

Size:96 Wells

Dog IL15 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	84	82-86
  1:2	93	90-95
  1:4	89	85-93
  1:8	105	101-109

• ELISA Kit Principle: Aviva Systems Biology IL15 ELISA Kit (Dog) (OKCA01045) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL15 captured in the well.
• ELISA Kit Range: 1.56 - 100 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	88	84-90
  EDTA Plasma (n=4)	100	97-103

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01045 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01045 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • IL15 Microplate: 96 Wells (12 x 8 Well strips)
  • IL15 Lyophilized Standard: 2 x 100 pg
  • 100X Biotinylated IL15 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.39 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: IL15
• Gene Full Name: Interleukin-15
• Alias Symbols: IL-15, MGC9721
• NCBI Gene Id: 443056
• Protein Name: Interleukin-15
• Description of Target: Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
• Uniprot ID: Q9XSJ6
• Protein Accession #: NP_001009734.1
• Nucleotide Accession #: NM_001009734.1