IL13RA1 ELISA Kit (Mouse) (OKCA01697)

Catalog No: OKCA01697

Size:96 Wells

Mouse IL13RA1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	105	97-109
  1:2	102	93-106
  1:4	97	85-100
  1:8	104	93-109

• ELISA Kit Principle: Aviva Systems Biology Il13ra1 ELISA Kit (Mouse) (OKCA01697) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il13ra1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il13ra1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Il13ra1 captured in the well.
• ELISA Kit Range: 31.25 - 2,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	105	97-109
  EDTA Plasma (n=4)	99	89-103

• ELISA Kit Component:
  • Il13ra1 Microplate: 96 Wells (12 x 8 Well strips)
  • Il13ra1 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated Il13ra1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 7.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: IL13RA1
• Gene Full Name: interleukin 13 receptor, alpha 1
• Alias Symbols: NR4, CD213a, Il13ra, CD213a1, AI882074, IL-13r[a], IL-13R-alpha-1
• NCBI Gene Id: 16164
• Protein Name: Interleukin-13 receptor subunit alpha-1
• Description of Target: Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
• Uniprot ID: O09030
• Protein Accession #: NP_598751.3
• Nucleotide Accession #: NM_133990.5