IL12RB1 ELISA Kit (Human) (OKCD00288)

Catalog No: OKCD00288 (Formerly GWB-KBBUX8)

Size:96WELLS

Human IL12RB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-91%	92-105%	81-97%	96-104%
  EDTA plasma(n=5)	79-102%	90-105%	97-105%	90-104%
  heparin plasma(n=5)	85-98%	82-91%	79-94%	96-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 Receptor Beta 1 (IL12Rb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 12 Receptor Beta 1 (IL12Rb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-101	91
  EDTA plasma(n=5)	91-104	99
  heparin plasma(n=5)	81-96	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12 Receptor Beta 1 (IL12Rb1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12 Receptor Beta 1 (IL12Rb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL12RB1 Microplate: 96 Wells (12 x 8 Well Strips)
  • IL12RB1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated IL12RB1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 12 Receptor Beta 1 (IL12Rb1). No significant cross-reactivity or interference between Interleukin 12 Receptor Beta 1 (IL12Rb1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL12RB1
• Gene Full Name: interleukin 12 receptor subunit beta 1
• Alias Symbols: CD212;cluster of differentiation 212;IL-12 receptor beta component;IL-12 receptor subunit beta-1;IL-12R subunit beta-1;IL12RB;IL-12R-BETA1;IMD30;interleukin 12 receptor, beta 1;interleukin-12 receptor beta-1 chain;interleukin-12 receptor subunit beta-1.
• NCBI Gene Id: 3594
• Protein Name: Interleukin-12 receptor subunit beta-1
• Description of Target: Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
• Uniprot ID: P42701
• Protein Accession #: NP_001276952
• Nucleotide Accession #: NM_001290023.1
• Protein Size (# AA): 662