IL12A ELISA Kit (Bovine) (OKEH03774)

Catalog No: OKEH03774

Size:96T

Bovine IL12A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IL12A ELISA Kit (Bovine) (OKEH03774) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL12A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL12A captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Anti-IL12A Microplate: 96 Wells (12 x 8 Well strips)
  • IL12A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated IL12A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.049 ng/mL
• Specificity: Natural and recombinant Bovine Interleukin-12 subunit alpha
• Publications:

  Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves. J Vet Med Sci. 83, 31-35 (2021). 33431726

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for IL12A ELISA Kit (Bovine) (OKEH03774) on CiteAb

Target Info

• Gene Symbol: IL12A
• Gene Full Name: interleukin 12A
• Alias Symbols: CLMF p35;cytotoxic lymphocyte maturation factor 35 kDa subunit;Il-12 p35;IL-12 subunit p35;IL-12A;IL12p35;interleukin 12 35kDa subunit;interleukin 12 polypeptide 35;interleukin-12 subunit alpha;natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35.
• NCBI Gene Id: 281856
• Protein Name: P54349
• Description of Target: Interleukin-12 subunit alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P54349
• Protein Accession #: NP_776780.1
• Nucleotide Accession #: NM_174355.2
• Protein Size (# AA): 221
• Molecular Weight: 25 kDa