IL10RB ELISA Kit (Human) (OKCD01201)

Catalog No: OKCD01201 (Formerly GWB-KBBLC1)

Size:96WELLS

Human IL10RB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	82-95%	96-104%	78-95%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 Receptor Beta (IL10Rb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 Receptor Beta (IL10Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 Receptor Beta (IL10Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 10 Receptor Beta (IL10Rb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	84-93	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 Receptor Beta (IL10Rb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 Receptor Beta (IL10Rb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL10RB Microplate: 96 Wells (12 x 8 Well Strips)
  • IL10RB Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated IL10RB Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 31 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 10 Receptor Beta (IL10Rb). No significant cross-reactivity or interference between Interleukin 10 Receptor Beta (IL10Rb) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL10RB
• Gene Full Name: interleukin 10 receptor subunit beta
• Alias Symbols: CDW210B;CRF2-4;CRFB4;cytokine receptor class-II CRF2-4;cytokine receptor class-II member 4;cytokine receptor family 2 member 4;cytokine receptor family II, member 4;D21S58;D21S66;IL-10 receptor subunit beta;IL-10R subunit 2;IL-10R subunit beta;IL-10R2;IL-10RB;interleukin 10 receptor, beta;interleukin-10 receptor subunit 2;interleukin-10 receptor subunit beta.
• NCBI Gene Id: 3588
• Protein Name: Interleukin-10 receptor subunit beta
• Description of Target: Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
• Uniprot ID: Q08334
• Protein Accession #: NP_000619
• Nucleotide Accession #: NM_000628.4
• Protein Size (# AA): 325