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0.1mg
$570.00
In Stock

Il10 Antibody (OASA08669)

Description of Target:
RABBIT ANTI RAT INTERLEUKIN-10
Gene Symbol:
Il10
NCBI Gene Id:
25325
Alias Symbols:
IL10X; Il10
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Il10.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Il10.
Swissprot Id:
P29456
Protein Accession #:
NP_036986.2
Host:
Rabbit
Protein Name:
Interleukin-10
Protein Size (# AA):
178
Specificity:
IL-10
Application:
ELISA, WB
Immunogen:
The immunogen for anti-Il10 antibody: recombinant rat IL-10
Product Format:
Purified IgG - lyophilised
Isotype:
Polyclonal IgG
Predicted Homology Based on Immunogen Sequence:
Rat
Species Reactivity:
Rat
Datasheets / Downloads:
Printable datasheet for
anti-Il10 antibody
- OASA08669
Applications Info:
ELISA:    0.5ug/ml
Western Blotting:    0.1ug/ml - 0.2ug/ml
Functional Assays:    0.5ug/ml - 0.9ug/ml
Additional Information:
Note: The addition of 0.09% sodium azide is recommended for long term storage.
N.B. For functional studies do not add sodium azide.
:::
Preservative Stabilisers: None present
Antiserum Preparation: Antisera to rat IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.1 ml distilled water
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Product Protocol: Il10 antibody used to evaluate functional and genetic analysis of two CD8 T cell subsets defined by the level of CD45RC expression in the rat (OASA08669)

Specificity: IL-10
Product Page: Il10 antibody (OASA08669)
Antibody Pair Available: OASA08669 Capture OASA08670 Detection
Experiment Type: Cytokine analysis

Protocol:
At various times throughout the culture (36 and 60 h for anti-TCR stimulation, 72, 96, and 120 h for MLR) supernatants were removed and stored at −20°C for cytokine determination; cells were harvested and RNA purified for analysis of lymphokine gene expression by RT-PCR. Emmanuel et. al. measured IFN-γ, IL-2, and IL-10 production in the supernatant by specific ELISA.

Summary:
In the present study, Emmanuel et. al. demonstrated for the first time that CD45RC expression distinguishes two CD8 T cell subsets in the rat: CDR45RChigh and CD45RClow. Upon in vitro stimulation in an APC-independent system, CD45RChigh CD8 T cells produce mainly IL-2 and IFN-γ, while the CD45RClow CD8 T cells produce only IL-4, IL-10, and IL-13.

References:
1: Xystrakis E, Cavailles P, Dejean AS, Cautain B, Colacios C, Lagrange D, van de
Gaar MJ, Bernard I, Gonzalez-Dunia D, Damoiseaux J, Fournié GJ, Saoudi A.
Functional and genetic analysis of two CD8 T cell subsets defined by the level of
CD45RC expression in the rat. J Immunol. 2004 Sep 1;173(5):3140-7. PubMed PMID:
15322174.

 
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