Catalog No: OKBB00190 (Formerly GWB-ZZD052)
Size:96 Tests
Price: $500.00
SKU
OKBB00190
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Mouse | |||||||||||||||||||||||||||||||||||
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Application | ELISA | |||||||||||||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | |||||||||||||||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | |||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva's mouse IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, F25-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL-6 amount of sample captured in plate. | |||||||||||||||||||||||||||||||||||
ELISA Kit Range | 15.6 pg/ml - 1,000 pg/ml | |||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
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ELISA Kit Component |
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Additional Information | Range: 15.6pg/ml-1000pg/ml | |||||||||||||||||||||||||||||||||||
:: | Principle: Aviva’s mouse IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse IL-6 specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-6 amount of sample captured in plate. | |||||||||||||||||||||||||||||||||||
:: | Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended. 2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case. 3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes. 4. Duplicate well assay is recommended for both standard and sample testing. 5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate. 6. Don’t reuse tips and tubes to avoid cross contamination. 7. To avoid to use the reagents from different batches together. 8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using. Background: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells.1 Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency.2 IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption.3 It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.4 Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline.5 BSF-2 is a novel interleukin consisting of 184 amino acids.6 The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa. | |||||||||||||||||||||||||||||||||||
Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | |||||||||||||||||||||||||||||||||||
Sample Type | cell culture supernates, serum and plasma (heparin, EDTA, citrate) | |||||||||||||||||||||||||||||||||||
Sensitivity | <1 pg/ml | |||||||||||||||||||||||||||||||||||
Predicted Homology Based on Immunogen Sequence | No detectable cross-reactivity with any other cytokine. | |||||||||||||||||||||||||||||||||||
Reference | 1. Bowcock, A. M.; Kidd, J. R.; Lathrop, G. M.; Daneshvar, L.; May, L. T.; Ray, A.; Sehgal, P. B.; Kidd, K. K.; Cavalli-Sforza, L. L. The human 'interferon-beta-2/hepatocyte stimulating factor/interleukin-6' gene: DNA polymorphism studies and localization to chromosome 7p21. Genomics 3: 8-16, 1988. 2. Ota, N.; Nakajima, T.; Nakazawa, I.; Suzuki, T.; Hosoi, T.; Orimo, H.; Inoue, S.; Shirai, Y.; Emi, M. A nucleotide variant in the promoter region of the interleukin-6 gene associated with decreased bone mineral density. J. Hum. Genet. 46: 267-272, 2001. 3. Chung, H. W.; Seo, J.-S.; Hur, S. E.; Kim, H. L.; Kim, J. Y.; Jung, J. H.; Kim, L. H.; Park, B. L.; Shin, H. D. Association of interleukin-6 promoter variant with bone mineral density in pre-menopausal women. J. Hum. Genet. 48: 243-248, 2003. 4. Ferrari, S. L.; Ahn-Luong, L.; Garnero, P.; Humphries, S. E.; Greenspan, S. L. Two promoter polymorphisms regulating interleukin-6 gene expression are associated with circulating levels of C-reactive protein and markers of bone resorption in postmenopausal women. J. Clin. Endocr. Metab. 88: 255-259, 2003. 5. Kiecolt-Glaser, J. K.; Preacher, K. J.; MacCullum, R. C.; Atkinson, C.; Malarkey, W. B.; Glaser, R. Chronic stress and age-related increases in the proinflammatory cytokine IL-6. Proc. Nat. Acad. Sci. 100: 9090-9095, 2003. 6. Hirano, T.; Yasukawa, K.; Harada, H.; Taga, T.; Watanabe, Y.; Matsuda, T.; Kashiwamura, S.; Nakajima, K.; Koyama, K.; Iwamatsu, A.; Tsunasawa, S.; Sakiyama, F.; Matsui, H.; Takahara, Y.; Taniguchi, T.; Kishimoto, T. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature 324: 73-76, 1986. | |||||||||||||||||||||||||||||||||||
Specificity | Natural and recombinant mouse IL-6 | |||||||||||||||||||||||||||||||||||
Immunogen | Expression system for standard: E.coli; Immunogen sequence: F25-T211 | |||||||||||||||||||||||||||||||||||
Description | For quantitative detection of mouse IL-6 in cell culture supernatants, serum and plasma( heparin, EDTA, citrate). |
Gene Symbol | IL6 |
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Gene Full Name | interleukin 6 |
Alias Symbols | B cell differentiation factor, B cell stimulatory factor 2, B-cell stimulatory factor 2, BSF 2, BSF-2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating fact, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFN-beta-2, IFNB2, IL 6, IL-6, IL6, IL6 protein, IL6_HUMAN, Interferon beta 2, Interferon beta-2, Interleukin 6, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6, Interleukin6 |
NCBI Gene Id | 16193 |
Protein Name | Interleukin-6 |
Description of Target | The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells.1 Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency.2 IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption.3 It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.4 Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline.5 BSF-2 is a novel interleukin consisting of 184 amino acids.6 |
Uniprot ID | P08505 |
Protein Accession # | NP_001300983.1 |
Nucleotide Accession # | NM_001314054.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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