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Catalog No: OKBB00380
Size:96 Wells
Price: $583.00
SKU
OKBB00380
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human IL-29 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-29 has been precoated onto 96-well plates. Standards(NS0, G20-T200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-29 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-29 amount of sample captured in plate.
ELISA Kit Range62.5 pg/ml - 4,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)5171446233664315502468
SD33.176.64114.545.01105.4140.7
CV%6.45.34.976.85.7
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human IL-29 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human IL-29 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human IL-29 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human IL-29
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: G20-T200
DescriptionFor quantitative detection of human IL-29 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolIFNL1
Gene Full Nameinterferon, lambda 1
Alias SymbolsCytokine ZCYTO21, IFN lambda 1, IFN-lambda-1, IFNL1, IL 29, IL-29, IL29, IL29_HUMAN, Interferon lambda 1, Interferon lambda-1, Interferon, lambda 1, Interleukin 29, Interleukin-29
NCBI Gene Id282618
Protein NameInterferon lambda-1
Description of TargetInterferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
Uniprot IDQ8IU54
Protein Accession #NP_742152.1
Nucleotide Accession #NM_172140.1
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