IL-2 ELISA Kit (Human) (OKBB00179)

Catalog No: OKBB00179

Size:96 Tests

Human IL-2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-2 has been precoated onto 96-well plates. Standards (E.coli,A21-T153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-2 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	75	272	537	96	310	643
  St. Dev.	3.45	9.52	16.65	6.048	16.12	39.2
  %CV	4.6	3.5	3.10	6.3	5.2	6.09

• ELISA Kit Component:
  • Lyophilized recombinant human IL-2 standard: 10 ng/tube x 2
  • Anti-human IL-2 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human IL-2 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 15.6pg/ml-1000pg/ml
  Principle: Aviva’s human IL-2 ELISA Kit was based on standard sandwich
  enzyme-linked immune-sorbent assay technology. Human IL-2 specific-specific
  monoclonal antibodies were precoated onto 96-well plates. The human specific
  detection polyclonal antibodies were biotinylated. The test samples and
  biotinylated detection antibodies were added to the wells subsequently and
  then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase
  Complex was added and unbound conjugates were washed away with PBS or
  TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction.
  TMB was catalyzed by HRP to produce a blue color product that changed into
  yellow after adding acidic stop solution. The density of yellow is proportional to
  the human IL-2 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3 Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <1 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Hollander, G. A.; Zuklys, S.; Morel, C.; Mizoguchi, E.; Mobisson, K.; Simpson, S.; Terhorst, C.; Wishart, W.; Golan, D.
  E.; Bhan, A. K.; Burakoff, S. J. Monoallelic expression of the interleukin-2 locus. Science 279: 2118-2121, 1998.
  2. Lowenthal, J. W.; Zubler, R. H.; Nabholz, M.; MacDonald, H. R. Similarities between interleukin-2 receptor number
  and affinity on activated B and T lymphocytes. Nature 315: 669-672, 1985.
  3. Shows, T.; Eddy, R.; Haley, L.; Byers, M.; Henry, M.; Fujita, T.; Matsui, H.; Taniguchi, T. Interleukin 2 (IL2) is
  assigned to human chromosome 4. Somat. Cell Molec. Genet. 10: 315-318, 1984.
• Specificity: Natural and recombinant human IL-2
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A21-T153
• Description: For quantitative detection of human IL-2 in cell culture supernatants, serum and plasma( heparin, EDTA, citrate).

Target Info

• Gene Symbol: IL2
• Gene Full Name: interleukin 2
• Alias Symbols: Aldesleukin, IL 2, IL-2, IL2, IL2_HUMAN, Interleukin 2, Interleukin-2, Interleukin2, Involved in regulation of T cell clonal expansion, Lymphokine, T cell growth factor, T-cell growth factor, TCGF
• NCBI Gene Id: 3558
• Protein Name: Interleukin-2
• Description of Target: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3 Human IL-2 is a 15.5 KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
• Uniprot ID: P60568
• Protein Accession #: NP_000577.2
• Nucleotide Accession #: NM_000586.3