Catalog No: OKBB00524
Size:96 Wells
Price: $583.00
SKU
OKBB00524
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse IL-17RC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17RC has been precoated onto 96-well plates. Standards(NS0, E22-R440) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL-17RC amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)145841126822163244737188
SD61.24267.3388.988.13335.5488.8
CV%4.26.55.75.47.56.8
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse IL-17RC Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse IL-17RC Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse IL-17RC Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse IL-17RC
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: E22-R440
DescriptionFor quantitative detection of mouse IL-17RC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolIL17RC
Gene Full Nameinterleukin 17 receptor C
Alias SymbolsIL 17 receptor C, IL 17RC, IL 17RL, IL17 receptor C, IL17F receptor, IL17RL, Interleukin 17 receptor C, Interleukin 17 receptor homolog, Interleukin 17 receptor like, Interleukin 17 receptor like protein
NCBI Gene Id171095
Protein NameInterleukin-17 receptor C
Description of TargetInterleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.
Uniprot IDQ8K4C2
Protein Accession #NP_598920.3
Nucleotide Accession #NM_134159.4
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