IL-10 High Sensitivity Kit (Human) (OKDB00042)

Catalog No: OKDB00042

Human IL-10 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Linearity: Two spiked human serum with different levels of IL-10 were analysed at three serial two fold dilutions (1:2-1:16) with two replicates each. Recoveries ranged from 90% to 117% with an overall mean recovery of 101%.
• ELISA Kit Principle: Aviva Systems Biology IL-10 High Sensitivity ELISA Kit (Human) (OKDB00042) is designed for the highly sensitive in-vitro quantitative measurement of recombinant or natural IL-10 in human serum, plasma or cell culture media. The kit is based on standard sandwich enzyme-linked immuno-sorbent assay methods along with an additional detection process employing a polymerization step to amplify the number of detector bound HRP molecules. A monoclonal antibody specific for IL-10 has been pre-coated into wells of a 96-well plate. Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for IL-10 is added, incubated and followed by washing. Streptavidin-HRP conjugate is then added, incubated and unbound conjugate is washed away. Amplification is achieved by adding a Biotin-Tyramine reagent, incubating and washing, followed by a second incubation with Streptavidin-HRP conjugate. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample captured in well.
• ELISA Kit Range: 1.56 - 50 pg/mL
• ELISA Kit Recovery: The spike recovery was evaluated by spiking two concentrations of recombinant IL-10 in human serum and culture media in three experiments. Recoveries ranged from 70% to 101% with an overall mean recovery of 85%.
• ELISA Kit Reproducibility: Four sera and one cell culture medium samples with various concentrations of IL-10 were tested for repeatability and reproducibility. Each assay was carried out with 3 replicates of each sample. Five independent assays were performed. The intra-assay and inter-assay coefficient of variation has been calculated to be 4.3% and 6.3% respectively.
• ELISA Kit Component:

  Reagents	1 x 48 Well Kit	1 x 96 Well Kit	2 x 96 Well Kit
  IL-10 Well Strip Microplate	6 x 8 Wells	12 x 8 Wells	24 x 8 Wells
  IL-10 Standard	1 Vial	2 Vials	4 Vials
  10X Standard Diluent (Buffer)	1 x 25 mL	1 x 25 mL	1 x 25 mL
  Standard Diluent (Serum)	1 x 7 mL	1 x 7 mL	2 x 7 mL
  27.5X Biotinylated anti-IL-10 Detector Antibody	1 x 400 uL	1 x 400 uL	2 x 400 uL
  Biotinylated Detector Antibody Diluent	1 x 7 mL	1 x 7 mL	1 x 13 mL
  Streptavidin-HRP	1 x 5 uL	2 x 5 uL	4 x 5 uL
  Amplification Diluent	1 x 25 mL	1 x 25 mL	1 x 25 mL
  100X Amplifier	1 x 200 uL	1 x 200 uL	2 x 200 uL
  HRP Diluent	1 x 25 mL	1 x 25 mL	2 x 25 mL
  200X Wash Buffer	1 x 10 mL	1 x 10 mL	2 x 10 mL
  TMB Substrate	1 x 11 mL	1 x 11 mL	2 x 24 mL

• Reconstitution and Storage: 2 to 8C, Do not use past expiration date.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: < 0.98 pg/mL
• Specificity: Ten specificities were tested with concentrations higher than IL-10 curve concentrations. No cross reaction was observed for concentrations for IL-1alpha and beta, IL-2, IL-5, IL-6, IL-8, IL-12, TNF-alpha, IFN-gamma and mIL-10.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL-10
• Gene Full Name: interleukin 10
• Alias Symbols: CSIF, TGIF, GVHDS, IL-10, IL10A
• NCBI Gene Id: 3586
• Protein Name: interleukin-10
• Description of Target: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
• Uniprot ID: P22301
• Protein Accession #: NP_000563.1
• Nucleotide Accession #: NM_00572.2
• Protein Size (# AA): 178
• Molecular Weight: 21 kDa