Catalog No: OKEH01391
Size:96T
Price: $525.00
SKU
OKEH01391
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology IKBKB ELISA Kit (Rat) (OKEH01391) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IKBKB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IKBKB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IKBKB captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.6% (n = 20) Mean Inter-assay CV%: < 7.5% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.156 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat Inhibitor of nuclear factor kappa-B kinase subunit beta | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Ikbkb |
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Gene Full Name | inhibitor of nuclear factor kappa B kinase subunit beta |
Alias Symbols | AIM-1;I-kappa-B kinase 2;I-kappa-B-kinase beta;IKK2;IKK-B;IKK-beta;inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta;inhibitor of kappaB kinase beta;inhibitor of nuclear factor kappa B kinase beta subunit;inhibitor of nuclear factor kappa-B kinase subunit beta;NFKBIKB;nuclear factor NF-kappa-B inhibitor kinase beta;serine/threonine protein kinase IKBKB. |
NCBI Gene Id | 84351 |
Protein Name | Q9QY78 |
Description of Target | Inhibitor of nuclear factor kappa-B kinase subunit beta |
Uniprot ID | https://www.uniprot.org/uniprot/Q9QY78 |
Protein Accession # | NP_445807.2 |
Nucleotide Accession # | NM_053355.2 |
Protein Size (# AA) | 757 |
Molecular Weight | 87 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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