Catalog No: OKEH00038
Size:96T
Price: $600.00
SKU
OKEH00038
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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IHH ELISA Kit (Human) : 96 Wells (OKEH00038)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00038
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IHH ELISA Kit (Human) (OKEH00038) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IHH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IHH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IHH captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
IHH Microplate96 Wells (12 x 8 Well strips)
IHH Lyophilized Standard2
100X Biotinylated IHH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.098 ng/mL
SpecificityNatural and recombinant Human Indian hedgehog protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIHH
Gene Full NameIndian hedgehog signaling molecule
Alias SymbolsBDA1;HHG2;HHG-2;Indian hedgehog homolog;indian hedgehog protein.
NCBI Gene Id3549
Protein NameQ14623
Description of TargetIndian hedgehog protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q14623
Protein Accession #NP_002172
Nucleotide Accession #NM_002181
Protein Size (# AA)411
Molecular Weight45 kDa
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