Catalog No: OKDD00330 (Formerly GWB-KBBTL8)
Size:96 Wells
Price: $650.00
SKU
OKDD00330
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
---|
Predicted Species Reactivity | Human | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology IgG3 ELISA Kit (Human) (OKDD00330) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for IgG3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IgG3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IgG3 captured in well. | ||||||||||||||||||||||||
ELISA Kit Range | 0.156-10 ng/mL | ||||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IgG3 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IgG3 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10%
Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates | ||||||||||||||||||||||||
Sensitivity | < 0.055 ng/mL | ||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of IgG3. No significant cross-reactivity or interference between IgG3 and analogues was observed. | ||||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review