Catalog No: OKEH03733
Size:96T
Price: $625.00
SKU
OKEH03733
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IGFBP7 ELISA Kit (Mouse) (OKEH03733) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igfbp7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Igfbp7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igfbp7 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Recovery<= 7.4%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.4% (n = 20)
Mean Inter-assay CV%: <= 9.7% (n = 20)
ELISA Kit Component
ComponentAmount
Igfbp7 Microplate96 Wells (12 x 8 Well strips)
Igfbp7 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Igfbp7 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.6 pg/mL
SpecificityNatural and recombinant Mouse Insulin-like growth factor-binding protein 7
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIgfbp7
Gene Full Nameinsulin-like growth factor binding protein 7
Alias SymbolsAG;AGM;angiomodulin;Fst;Fstl2;IBP-7;IGF-binding protein 7;IGFBP-7;insulin-like growth factor-binding protein 7;mac2;Mac25;MAC25 protein.
NCBI Gene Id29817
Protein NameQ61581
Description of TargetInsulin-like growth factor-binding protein 7
Uniprot IDhttps://www.uniprot.org/uniprot/Q61581
Protein Accession #NP_032074.3
Nucleotide Accession #NM_008048.3
Protein Size (# AA)313
Molecular Weight33 kDa
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