IGFBP7 ELISA Kit (Human) (OKBB00381)

Catalog No: OKBB00381

Size:96 Wells

Human IGFBP7 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human IGFBP7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IGFBP7 has been precoated onto 96-well plates. Standards(NS0, D30-L282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IGFBP7 amount of sample captured in plate.
• ELISA Kit Range: 625 pg/ml - 40,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	2.3	16.2	28.1	2.8	15.4	29.7
  SD	0.101	0.729	1.43	0.188	1.063	2.14
  CV%	4.4	4.5	5.1	6.7	6.9	7.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human IGFBP7 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human IGFBP7 Standard: 2 x 20 ng/tube
  • 100X Biotinylated Anti-Human IGFBP7 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
• Sensitivity: <20 pg/ml
• Specificity: Natural and recombinant human IGFBP7
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: D30-L282
• Description: For quantitative detection of human IGFBP7 in cell culture supernatants, serum, plasma(heparin, EDTA) and urine.

Target Info

• Gene Symbol: IGFBP7
• Gene Full Name: insulin-like growth factor binding protein 7
• Alias Symbols: AGM, Angiomodulin, FSTL2, IBP-7, IBP7, IBP7_HUMAN, IGF binding protein 7, IGF-binding protein 7, IGFBP rP1, IGFBP-7, IGFBP-rP1, IGFBP7, IGFBPRP1, Insulin like growth factor binding protein 7, Insulin-like growth factor-binding protein 7, MAC25, MAC25 protein, PGI2 stimulating factor, PGI2-stimulating factor, Prostacyclin stimulating factor, Prostacyclin-stimulating factor, PSF, RAMSVPS, TAF, Tumor-derived adhesion factor
• NCBI Gene Id: 3490
• Protein Name: Insulin-like growth factor-binding protein 7
• Description of Target: Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.
• Uniprot ID: Q16270
• Protein Accession #: NP_001240764.1
• Nucleotide Accession #: NM_001253835.1