Catalog No: OKBB00478
Size:96 Wells
Price: $583.00
SKU
OKBB00478
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human IGFBP6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP6 has been precoated onto 96-well plates. Standards(NS0, A25-G240) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IGFBP6 amount of sample captured in plate.
ELISA Kit Range62.5 pg/ml - 4,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)5511557273648916352878
SD21.563.84128.627.87101.37184.2
CV%3.94.14.75.76.26.4
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human IGFBP6 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human IGFBP6 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human IGFBP6 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human IGFBP6
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: A25-G240
DescriptionFor quantitative detection of human IGFBP6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolIGFBP6
Gene Full Nameinsulin-like growth factor binding protein 6
Alias SymbolsIBP 6, IBP-6, IBP6, IBP6_HUMAN, IGF binding protein 6, IGF-binding protein 6, IGFBP 6, IGFBP-6, IGFBP6, Insulin like growth factor binding protein 6, Insulin-like growth factor-binding protein 6
NCBI Gene Id3489
Protein NameInsulin-like growth factor-binding protein 6
Description of TargetInsulin-like growth factor-binding protein 6 (IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.
Uniprot IDP24592
Protein Accession #NP_002169.1
Nucleotide Accession #NM_002178.2
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