IGFBP6 ELISA Kit (Bovine) (OKCD02622)

Catalog No: OKCD02622

Size:96WELLS

Bovine IGFBP6 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-93%	79-99%	88-102%	92-99%
  EDTA Plasma (n=5)	91-99%	86-99%	94-101%	80-101%
  Heparin Plasma (n=5)	80-95%	85-105%	92-105%	93-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 6 (IGFBP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 6 (IGFBP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 6 (IGFBP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Like Growth Factor Binding Protein 6 (IGFBP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 2.50-160ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-93	90
  EDTA Plasma (n=5)	85-99	93
  Heparin Plasma(n=5)	78-89	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 6 (IGFBP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 6 (IGFBP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IGFBP6 Microplate: 96 Wells (12 x 8 Well strips)
  • IGFBP6 Lyophilized Standard: 2
  • 100X Biotinylated IGFBP6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.94 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 6 (IGFBP6). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 6 (IGFBP6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGFBP6
• Gene Full Name: insulin like growth factor binding protein 6
• Alias Symbols: IBP-6;IGF-binding protein 6;IGFBP-6;insulin-like growth factor-binding protein 6.
• NCBI Gene Id: 404186
• Protein Name: Insulin-like growth factor-binding protein 6
• Description of Target: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
• Uniprot ID: Q05718
• Protein Accession #: NP_001035585.1
• Nucleotide Accession #: NM_001040495.2
• Protein Size (# AA): 237