Catalog No: OKBB00525
Size:96 Wells
Price: $583.00
SKU
OKBB00525
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP5 has been precoated onto 96-well plates. Standards(NS0, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IGFBP5 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)1.13.46.21.53.86.7
SD0.0450.1670.3410.0840.2470.476
CV%4.14.95.55.66.57.1
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse IGFBP5 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse IGFBP5 Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse IGFBP5 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse IGFBP5
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: L20-E271
DescriptionFor quantitative detection of mouse IGFBP5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolIGFBP5
Gene Full Nameinsulin-like growth factor binding protein 5
Alias SymbolsIBP 5, IBP-5, IBP5, IBP5_HUMAN, IGF binding protein 5, IGF BP5, IGF-binding protein 5, IGFBP 5, IGFBP-5, IGFBP5, Insulin like growth factor binding protein 5, Insulin-like growth factor-binding protein 5
NCBI Gene Id16011
Protein NameInsulin-like growth factor-binding protein 5
Description of TargetInsulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.
Uniprot IDQ07079
Protein Accession #NP_034648.2
Nucleotide Accession #NM_010518.2
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