Catalog No: OKEH03930
Size:96T
Price: $625.00
SKU
OKEH03930
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IGFBP5 ELISA Kit (Bovine) (OKEH03930) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP5 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Component
ComponentAmount
IGFBP5 Microplate96 Wells (12 x 8 Well strips)
IGFBP5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated IGFBP5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.28 ng/mL
SpecificityNatural and recombinant Bovine Insulin-like growth factor-binding protein 5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIGFBP5
Gene Full Nameinsulin like growth factor binding protein 5
Alias SymbolsIBP-5;IGF-binding protein 5;IGFBP-5;insulin-like growth factor-binding protein 5.
NCBI Gene Id404185
Protein NameQ05717
Description of TargetInsulin-like growth factor-binding protein 5
Uniprot IDhttps://www.uniprot.org/uniprot/Q05717
Protein Accession #NP_001098797.1
Nucleotide Accession #NM_001105327.2
Protein Size (# AA)271
Molecular Weight271 kDa
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