IGFBP3 ELISA Kit (Human) : 96 Wells (OKEH00085)

Catalog No: OKEH00085

Size:96T

Human IGFBP3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IGFBP3 ELISA Kit (Human) (OKEH00085) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP3 captured in well.
• ELISA Kit Range: 125-8000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 7.6% (n = 20)
  Mean Inter-assay CV%: < 8.5% (n = 20)
• ELISA Kit Component:
  • IGFBP3 Microplate: 96 Wells (12 x 8 Well strips)
  • IGFBP3 Lyophilized Standard: 2
  • 100X Biotinylated IGFBP3 Detector Antibody: 1 x 20 mL
  • 100X Avidin-HRP Conjugate: 1 x 60 uL
  • Sample Diluent: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 50 pg/mL
• Specificity: Natural and recombinant Human Insulin-like growth factor-binding protein 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGFBP3
• Gene Full Name: insulin like growth factor binding protein 3
• Alias Symbols: acid stable subunit of the 140 K IGF complex;binding protein 29;binding protein 53;BP-53;growth hormone-dependent binding protein;IBP3;IBP-3;IGF-binding protein 3;IGFBP-3;insulin-like growth factor-binding protein 3.
• NCBI Gene Id: 3486
• Protein Name: P17936
• Description of Target: Insulin-like growth factor-binding protein 3
• Uniprot ID: https://www.uniprot.org/uniprot/P17936
• Protein Accession #: NP_000589
• Nucleotide Accession #: NM_000598
• Protein Size (# AA): 291
• Molecular Weight: 32 kDa