IGFBP2 ELISA Kit (Human) (OKBB00397)

Catalog No: OKBB00397

Size:96 Wells

Human IGFBP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human IGFBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP2 has been precoated onto 96-well plates. Standards(NS0, E40-Q328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IGFBP2 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	608	1555	2836	715	1938	3147
  SD	27.97	80.86	167.3	44.33	133.7	229.7
  CV%	4.6	5.2	5.9	6.2	6.9	7.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human IGFBP2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human IGFBP2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human IGFBP2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human IGFBP2
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E40-Q328
• Description: For quantitative detection of human IGFBP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Target Info

• Gene Symbol: IGFBP2
• Gene Full Name: insulin-like growth factor binding protein 2, 36kDa
• Alias Symbols: BP 2, BP2, IBP 2, IBP-2, IBP2, IBP2_HUMAN, IGF binding protein 2, IGF BP53, IGF-binding protein 2, IGF-BP53, IGFBP-2, IGFBP2, IGFBP53, Insulin Like Growth Factor Binding Protein 2, Insulin like growth factor binding protein 2 36kDa, Insulin like growth factor-binding protein 2 precursor, Insulin-like growth factor-binding protein 2
• NCBI Gene Id: 3485
• Protein Name: Insulin-like growth factor-binding protein 2
• Description of Target: Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways is regulators of cancer-mediated endothelial recruitment.
• Uniprot ID: P18065
• Protein Accession #: NP_000588.2
• Nucleotide Accession #: NM_000597.2