Catalog No: OKEH00083
Size:96T
Price: $325.00
SKU
OKEH00083
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

IGFBP1 ELISA Kit (Human) : 96 Wells (OKEH00083)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IGFBP1 ELISA Kit (Human) (OKEH00083) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP1 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 8.4% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
IGFBP1 Microplate96 Wells (12 x 8 Well strips)
IGFBP1 Lyophilized Standard2
100X Biotinylated IGFBP1 Detector Antibody1 x 20 mL
100X Avidin-HRP Conjugate1 x 60 uL
Sample Diluent1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity10.2 pg/mL
SpecificityNatural and recombinant Human Insulin-like growth factor-binding protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIGFBP1
Gene Full Nameinsulin like growth factor binding protein 1
Alias SymbolsAFBP;alpha-pregnancy-associated endometrial globulin;amniotic fluid binding protein;binding protein-25;binding protein-26;binding protein-28;growth hormone independent-binding protein;hIGFBP-1;IBP1;IBP-1;IGF-binding protein 1;IGFBP-1;IGF-BP25;insulin-like growth factor-binding protein 1;placental protein 12;PP12.
NCBI Gene Id3484
Protein NameP08833
Description of TargetInsulin-like growth factor-binding protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/P08833
Protein Accession #NP_000587
Nucleotide Accession #NM_000596
Protein Size (# AA)259
Molecular Weight28 kDa
Write Your Own Review
You're reviewing:IGFBP1 ELISA Kit (Human) : 96 Wells (OKEH00083)
Your Rating
We found other products you might like!