IGFBP-3 ELISA Kit (Mouse) (OKBB00172)

Catalog No: OKBB00172

Size:96 Tests

Mouse IGFBP3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse IGFBP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP-3 has been precoated onto 96-well plates. Standards(NS0, G28Q-K291) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IGFBP-3 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (ng/mL)	1.22	3.83	6.21	1.38	3.75	6.07
  St. Dev.	0.066	0.18	0.404	0.104	0.308	0.443
  %CV	5.40	4.69	6.50	7.53	8.21	7.29

• ELISA Kit Component:
  • Lyophilized recombinant mouse Fractalkine standard: 10 ng/tube x 2
  • Anti-mouse Fractalkine Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-mouse Fractalkine antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 156pg/ml-10,000pg/ml
  Principle: Aviva’s mouse IGFBP-3 ELISA Kit was based on standard sandwich
  enzyme-linked immune-sorbent assay technology. Mouse IGFBP-3
  specific-specific polyclonal antibodies were precoated onto 96-well plates. The
  mouse specific detection polyclonal antibodies were biotinylated. The test
  samples and biotinylated detection antibodies were added to the wells
  subsequently and then followed by washing with PBS or TBS buffer.
  Avidin-Bioyin-Peroxidase Complex was added and unbound conjugates were
  washed away with PBS or TBS buffer. HRP substrate TMB was used to
  visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a
  blue color product that changed into yellow after adding acidic stop solution.
  The density of yellow is proportional to the mouse IGFBP-3 amount of sample
  captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression.1 Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes.2 IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix.3 IGFBP3 is also located on chromosome 7.4 The standard product used in this kit is recombinant mouse IGFBP-3, consisting of 270 amino acids with the molecular mass of 29.4kDa. As a result of glycosylation, the molecular mass is 40-50KDa.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates and serum
• Sensitivity: <10 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Deal, C.; Ma, J.; Wilkin, F.; Paquette, J.; Rozen, F.; Ge, B.; Hudson, T.; Stampfer, M.; Pollak, M. Novel promoter polymorphism in insulin-like
  growth factor-binding protein-3: correlation with serum levels and interaction with known regulators. J. Clin. Endocr. Metab. 86: 1274-1280,
  2001.
  2. Spagnoli, A.; Torello, M.; Nagalla, S. R.; Horton, W. A.; Pattee, P.; Hwa, V.; Chiarelli, F.; Roberts, C. T., Jr.; Rosenfeld, R. G. Identification of
  STAT-1 as a molecular target of IGFBP-3 in the process of chondrogenesis. J. Biol. Chem. 277: 18860-18867, 2002.3. Weinzimer, S. A.; Gibson, T. B.; Collett-Solberg, P. F.; Khare, A.; Liu, B.; Cohen, P. Transferrin is an insulin-like growth factor-binding
  protein-3 binding protein. J. Clin. Endocr. Metab. 86: 1806-1813, 2001.
  4. Ehrenborg, E.; Larsson, C.; Stern, I.; Janson, M.; Powell, D. R.; Luthman, H. Contiguous localization of the genes encoding mouse
  insulin-like growth factor-binding proteins 1 (IGBP1) and 3 (IGBP3) on chromosome 7. Genomics 12: 497-502, 1992.
• Specificity: Natural and recombinant mouse IGFBP-3
• Immunogen: Expression system for standard: NS0; Immunogen sequence: G28Q-K291
• Description: For quantitative detection of mouse IGFBP-3 in cell culture supernatants and serum.

Target Info

• Gene Symbol: IGFBP3
• Gene Full Name: insulin-like growth factor binding protein 3
• Alias Symbols: Acid stable subunit of the 140 K IGF complex, Binding protein 29, Binding protein 53, BP 53, BP53, Growth hormone dependent binding protein, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, Insulin Like Growth Factor Binding Protein 3
• NCBI Gene Id: 3486
• Protein Name: Insulin-like growth factor-binding protein 3
• Description of Target: Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression.1 Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes.2 IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix.3 IGFBP3 is also located on chromosome 7.4 The standard product used in this kit is recombinant mouse IGFBP-3, consisting of 270 amino acids with the molecular mass of 29.4kDa. As a result of glycosylation, the molecular mass is 40-50KDa.
• Uniprot ID: P47878
• Protein Accession #: NP_032369.2
• Nucleotide Accession #: NM_008343.2