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Catalog No: OKEH04275
Size:96T
Price: $525.00
SKU
OKEH04275
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Igf2r ELISA Kit (Mouse) (OKEH04275) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igf2r has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Igf2r is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igf2r captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.8% (n = 20)
Mean Inter-assay CV%: <= 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
Igf2r Microplate96 Wells (12 x 8 Well Strips)
Igf2r Standard2 x 2 ng
Sample Diluent1 x 20 mL
Igf2r Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.087 ng/mL
SpecificityNatural and recombinant Mouse Cation-independent mannose-6-phosphate receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIgf2r
Gene Full Nameinsulin-like growth factor 2 receptor
Alias Symbols300 kDa mannose 6-phosphate receptor;AI661837;cation-independent mannose-6-phosphate receptor;cation-independent MPR;CD222;CI-;CI Man-6-P receptor;CI-MPR;IGF-II receptor;IGF-II/CI-MPR;Insulin-like growth factor 2 receptor;insulin-like growth factor II receptor;M6P/IG;M6P/IGF2 receptor;M6P/IGF2R;M6PR;mannose-6-phosphate receptor, cation independent;MPR 300;Mpr3;Mpr300.
NCBI Gene Id16004
Protein NameQ07113
Description of TargetCation-independent mannose-6-phosphate receptor
Uniprot IDhttps://www.uniprot.org/uniprot/Q07113
Protein Accession #NP_034645.2
Nucleotide Accession #NM_010515.2
Protein Size (# AA)2483
Molecular Weight274 kDa
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