IGF2 ELISA Kit (Rat) (OKEH04126)

Catalog No: OKEH04126

Size:96T

Rat IGF2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Igf2 ELISA Kit (Rat) (OKEH04126) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igf2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Igf2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igf2 captured in the well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.2% (n = 20)
  Mean Inter-assay CV%: <= 8.6% (n = 20)
• ELISA Kit Component:
  • Anti-Igf2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Igf2 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Igf2 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 15.66 pg/mL
• Specificity: Natural and recombinant Rat Insulin-like growth factor II
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Igf2
• Gene Full Name: insulin-like growth factor 2
• Alias Symbols: IGFII;IGF-II;insulin-like growth factor II;insulin-like growth factor II (IGF-II);Insulin-like growth factor II (somatomedin A);MSA;multiplication-stimulating activity;multiplication-stimulating polypeptide;RNIGF2.
• NCBI Gene Id: 24483
• Protein Name: P01346
• Description of Target: Insulin-like growth factor II
• Uniprot ID: https://www.uniprot.org/uniprot/P01346
• Protein Accession #: NP_001177091.1
• Nucleotide Accession #: NM_001190162.1
• Protein Size (# AA): 180
• Molecular Weight: 20 kDa