IgE ELISA Kit (Mouse) (OKEH03652)

Catalog No: OKEH03652

Size:96T

Mouse IGE ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IgE ELISA Kit (Mouse) (OKEH03652) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IgE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for IgE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgE captured in well.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.3% (n = 20)
  Mean Inter-assay CV%: <= 6.5% (n = 20)
• ELISA Kit Component:
  • IgE Microplate: 96 Wells (12 x 8 Well strips)
  • IgE Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated IgE Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.65 ng/mL
• Specificity: Natural and recombinant Mouse Immunoglobulin E
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGE