IgE ELISA Kit (Human) : 96 Wells (OKEH00514)

Catalog No: OKEH00514

Size:96T

Bovine IgE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IgE ELISA Kit (Human) (OKEH00514) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bovine IgE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Bovine IgE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bovine IgE captured in well.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 11.5% (n = 20)
• ELISA Kit Component:
  • Anti-IgE Microplate: 96 Wells (12 x 8 Well strips)
  • IgE Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated IgE: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.78 ng/mL
• Specificity: Natural and recombinant Human Immunoglobulin E
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGE
• Description of Target: Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are only 0.05% of the Ig concentration, compared to 10?mg/ml for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful inflammation reactions.