IFNAR1 ELISA Kit (Human) (OKEH04857)

Catalog No: OKEH04857

Size:96T

Human IFNAR1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology IFNAR1 ELISA Kit (Human) (OKEH04857) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFNAR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFNAR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFNAR1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay Precision - < 4.4%, n = 20
  Mean Inter-Assay Precision - < 6.7%, n = 20
• ELISA Kit Component:
  • IFNAR1 Microplate: 96 Wells (12 x 8 Well Strips)
  • IFNAR1 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • IFNAR1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.034 ng/mL
• Specificity: Natural and recombinant Human Interferon alpha/beta receptor 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IFNAR1
• Gene Full Name: interferon alpha and beta receptor subunit 1
• Alias Symbols: alpha-type antiviral protein;AVP;beta-type antiviral protein;CRF2-1;cytokine receptor class-II member 1;cytokine receptor family 2 member 1;IFNalpha/beta receptor 1;IFN-alpha/beta receptor 1;IFN-alpha-REC;IFNAR;IFNBR;IFN-R-1;IFRC;interferon (alpha, beta and omega) receptor 1;interferon alpha/beta receptor 1;interferon receptor 1;interferon-alpha/beta receptor alpha chain;interferon-beta receptor 1;type I interferon receptor 1.
• NCBI Gene Id: 3454
• Protein Name: P17181
• Description of Target: Interferon alpha/beta receptor 1
• Uniprot ID: https://www.uniprot.org/uniprot/P17181
• Protein Accession #: NP_000620.2
• Nucleotide Accession #: NM_000629.2
• Protein Size (# AA): 557
• Molecular Weight: 64 kDa