Catalog No: OKEH01738
Size:96T
Price: $600.00
SKU
OKEH01738
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology IFI44 ELISA Kit (Human) (OKEH01738) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human IFI44 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated IFI44 and incubated. The IFI44 found in the sample or standards competes with the biotinylated IFI44 for limited binding sites on the immobilized anti-human IFI44 antibody. Excess unbound biotinylated IFI44 and sample or standard IFI44 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated IFI44 captured in the well and inversely proportional to the amount of IFI44 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.5% (n = 20) Mean Inter-assay CV%: < 8.2% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.29 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Interferon-induced protein 44 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | IFI44 |
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Gene Full Name | interferon induced protein 44 |
Alias Symbols | interferon-induced protein 44;interferon-induced, hepatitis C-associated microtubular aggregate protein (44kD);microtubule-associated protein 44;MTAP44;p44;TBC/LysM-associated domain containing 5;TLDC5. |
NCBI Gene Id | 10561 |
Protein Name | Q8TCB0 |
Description of Target | Interferon-induced protein 44 |
Uniprot ID | https://www.uniprot.org/uniprot/Q8TCB0 |
Protein Accession # | NP_006408.3 |
Nucleotide Accession # | NM_006417.4 |
Protein Size (# AA) | 444 |
Molecular Weight | 50 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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