Catalog No: OKCD00770 (Formerly GWB-KBBNO9)
Size:96WELLS
Price: $700.00
SKU
OKCD00770
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Datasheets/ManualsPrintable datasheet for IDO2 ELISA Kit (Mouse) (OKCD00770)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-99%86-94%86-99%78-93%
EDTA plasma(n=5)91-105%94-101%88-98%81-94%
heparin plasma(n=5)87-97%81-101%79-104%86-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Indoleamine-2,3-Dioxygenase 2 (IDO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Indoleamine-2,3-Dioxygenase 2 (IDO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Indoleamine-2,3-Dioxygenase 2 (IDO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Indoleamine-2,3-Dioxygenase 2 (IDO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.25-2000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)95-10299
EDTA plasma(n=5)82-9590
heparin plasma(n=5)82-9188
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Indoleamine-2,3-Dioxygenase 2 (IDO2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indoleamine-2,3-Dioxygenase 2 (IDO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Ido2 Microplate96 Wells (12 x 8 Well Strips)
Ido2 Lyophilized Standard2 x 2 ng
100X Biotinylated Ido2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 12.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Indoleamine-2,3-Dioxygenase 2 (IDO2). No significant cross-reactivity or interference between Indoleamine-2,3-Dioxygenase 2 (IDO2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIdo2
Gene Full Nameindoleamine 2,3-dioxygenase 2
Alias SymbolsAI265623;C230043N17Rik;Id;Ido-2;In;Indol1;indoleamine 2,3-dioxygenase 2;indoleamine 2,3-dioxygenase-like protein 1;indoleamine-pyrrole 2,3 dioxygenase-like 1;Indoleamine-pyrrole 2,3-dioxygenase-like protein 1.
NCBI Gene Id209176
Protein NameIndoleamine 2,3-dioxygenase 2
Description of TargetCatalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism (PubMed:17499941, PubMed:17499941). Involved in immune regulation (PubMed:25477879).
Uniprot IDQ8R0V5
Protein Accession #NP_666061
Nucleotide Accession #NM_145949.2
Protein Size (# AA)405
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