Catalog No: OKCD02559
Size:96WELLS
Price: $700.00
SKU
OKCD02559
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Datasheets/ManualsPrintable datasheet for IDE ELISA Kit (Rat) (OKCD02559)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Degrading Enzyme (IDE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Degrading Enzyme (IDE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Degrading Enzyme (IDE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Degrading Enzyme (IDE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Degrading Enzyme (IDE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Degrading Enzyme (IDE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IDE Microplate96 Wells (12 x 8 Well strips)
IDE Lyophilized Standard2
100X Biotinylated IDE Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.124 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin Degrading Enzyme (IDE). No significant cross-reactivity or interference between Insulin Degrading Enzyme (IDE) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolIde
Gene Full Nameinsulin degrading enzyme
Alias SymbolsINSDEGM;insulin protease;insulinase;insulin-degrading enzyme;insulysin.
NCBI Gene Id25700
Protein NameInsulin-degrading enzyme
Description of TargetPlays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP.
Uniprot IDP35559
Protein Accession #NP_037291.1
Nucleotide Accession #NM_013159.1
Protein Size (# AA)1019
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