ICTP ELISA Kit (Human) (OKEH00680)

Catalog No: OKEH00680

Size:96T

Human ICTP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ICTP ELISA Kit (Human) (OKEH00680) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ICTP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ICTP and incubated. The ICTP found in the sample or standards competes with the biotinylated ICTP for limited binding sites on the immobilized anti-human ICTP antibody. Excess unbound biotinylated ICTP and sample or standard ICTP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ICTP captured in the well and inversely proportional to the amount of ICTP which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.4% (n = 20)
  Mean Inter-assay CV%: < 8.1% (n = 20)
• ELISA Kit Component:
  • Anti-ICTP Microplate: 96 Wells (12 x 8 Well strips)
  • ICTP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X ICTP-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.044 ng/mL
• Specificity: Natural and recombinant Human Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain
• Publications:

  Oral Fluid Biomarkers for Diagnosing Gingivitis in Human: A Cross-Sectional Study. J Clin Med. 9, NULL (2020). 32503210

• Assay Info: Assay Methodology: Quantitative Competitive ELISA
• Description:
  View product citations for ICTP ELISA Kit (Human) (OKEH00680) on CiteAb

Target Info

• Gene Symbol: ICTP
• Alias Symbols: Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain
• Protein Name: Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain
• Description of Target: In bone physiology, the C-terminal telopeptide (or more formally, carboxy-terminal collagen crosslinks, and known by the acronym CTX) is a serum biomarker used to measure the rate of bone turnover. It can be useful in assisting clinicians to determine a patient's nonsurgical treatment response as well as evaluate a patient's risk of developing complications during healing following surgical intervention. The test used to detect the CTX marker is called the Serum CrossLaps, and it is more specific to bone resorption than any other test currently available.