ICOS ELISA Kit (Human) (OKCD00111)

Catalog No: OKCD00111 (Formerly GWB-KBBII5)

Size:96WELLS

Human ICOS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-92%	79-89%	84-101%	79-98%
  EDTA plasma(n=5)	98-105%	85-92%	79-97%	78-93%
  heparin plasma(n=5)	89-101%	78-94%	94-101%	82-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inducible T-Cell Co Stimulator (ICOS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inducible T-Cell Co Stimulator (ICOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inducible T-Cell Co Stimulator (ICOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Inducible T-Cell Co Stimulator (ICOS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	91-98	94
  EDTA plasma(n=5)	90-97	94
  heparin plasma(n=5)	80-103	81

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inducible T-Cell Co Stimulator (ICOS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inducible T-Cell Co Stimulator (ICOS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ICOS Microplate: 96 Wells (12 x 8 Well Strips)
  • ICOS Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated ICOS Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, cell lysates and other biological fluids
• Sensitivity: < 5.8 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Inducible T-Cell Co Stimulator (ICOS). No significant cross-reactivity or interference between Inducible T-Cell Co Stimulator (ICOS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ICOS
• Gene Full Name: inducible T cell costimulator
• Alias Symbols: activation-inducible lymphocyte immunomediatory molecule;AILIM;CD278;CVID1;inducible costimulator;inducible T-cell costimulator;inducible T-cell co-stimulator.
• NCBI Gene Id: 29851
• Protein Name: Inducible T-cell costimulator
• Description of Target: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).
• Uniprot ID: Q9Y6W8
• Protein Accession #: NP_036224
• Nucleotide Accession #: NM_012092.3
• Protein Size (# AA): 199