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Catalog No: OKCD02608
Size:96WELLS
Price: $650.00
SKU
OKCD02608
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Datasheets/ManualsPrintable datasheet for ICAM4 ELISA Kit (Mouse) (OKCD02608)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
97-105%78-90%79-101%83-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 4 (ICAM4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 4 (ICAM4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 4 (ICAM4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Intercellular Adhesion Molecule 4 (ICAM4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
90-10497
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 4 (ICAM4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 4 (ICAM4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ICAM4 Microplate96 Wells (12 x 8 Well strips)
ICAM4 Lyophilized Standard2
100X Biotinylated ICAM4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 4 (ICAM4). No significant cross-reactivity or interference between Intercellular Adhesion Molecule 4 (ICAM4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolIcam4
Gene Full Nameintercellular adhesion molecule 4, Landsteiner-Wiener blood group
Alias Symbols1810015M19Rik;Cd242;ICAM-4;intercellular adhesion molecule 4;LW glycoprotein.
NCBI Gene Id78369
Protein NameIntercellular adhesion molecule 4
Description of TargetAdhesion molecule that binds to leukocyte adhesion LFA-1 protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins (By similarity). Isoform 2 may modulate binding of membrane-associated ICAM4.
Uniprot IDQ9ERM2
Protein Accession #NP_076381.1
Nucleotide Accession #NM_023892.2
Protein Size (# AA)262
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