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Catalog No: OKEH04482
Size:96T
Price: $525.00
SKU
OKEH04482
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ICAM4 ELISA Kit (Human) (OKEH04482) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ICAM4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ICAM4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ICAM4 captured in the well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.9% (n = 20)
Mean Inter-assay CV%: <= 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ICAM4 Microplate96 Wells (12 x 8 Well Strips)
ICAM4 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
ICAM4 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.78 ng/mL
SpecificityNatural and recombinant Human Intercellular adhesion molecule 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolICAM4
Gene Full Nameintercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
Alias SymbolsCD242;CD242 antigen;intercellular adhesion molecule 4;intercellular adhesion molecule 4 (LW blood group);Landsteiner-Wiener blood group antigen a;Landsteiner-Wiener blood group glycoprotein;LW;LW antigen a.
NCBI Gene Id3386
Protein NameQ14773
Description of TargetIntercellular adhesion molecule 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q14773
Protein Accession #NP_001034221.1
Nucleotide Accession #NM_001039132.2
Protein Size (# AA)271
Molecular Weight29 KDa
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