ICAM-3 ELISA Kit (Human) (OKBB00468)

Catalog No: OKBB00468

Size:96 Wells

Human ICAM-3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human ICAM-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-3 has been precoated onto 96-well plates. Standards(NS0, Q30-H485, homodimer) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ICAM-3 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	0.58	2.73	5.45	0.68	3.16	6.24
  SD	0.021	0.123	0.294	0.04	0.215	0.443
  CV%	3.7	4.5	5.4	5.9	6.8	7.1

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human ICAM-3 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human ICAM-3 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human ICAM-3 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human ICAM-3
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q30-H485, homodimer
• Description: For quantitative detection of human ICAM-3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: ICAM3
• Gene Full Name: intercellular adhesion molecule 3
• Alias Symbols: CD50, CD50 antigen, CDW50, ICAM 3, ICAM R, ICAMR, Intercellular adhesion molecule 3, Intercellular adhesion molecule 3 precursor
• NCBI Gene Id: 3385
• Protein Name: Intercellular adhesion molecule 3
• Description of Target: Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.
• Uniprot ID: P32942
• Protein Accession #: NP_002153.2
• Nucleotide Accession #: NM_002162.3