Catalog No: OKBB00159 (Formerly GWB-ZZD130)
Size:96 Tests
Price: $583.00
SKU
OKBB00159
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's mouse ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ICAM-1 has been precoated onto 96-well plates. Standards(NS0, Q28-N485) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse ICAM-1 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)104637796637114544766450
St. Dev.47.07215.4404.961.83282464.4
%CV4.55.696.105.46.307.2
ELISA Kit Component
ComponentAmount
Lyophilized recombinant mouse Fractalkine standard10 ng/tube x 2
Anti-mouse Fractalkine Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-mouse Fractalkine antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
Predicted Homology Based on Immunogen SequenceNo detectable cross-reactivity with any other cytokine.
Reference1. Ballantyne, C. M.; Kozak, C. A.; O'Brien, W. E.; Beaudet, A. L. Assignment of the gene for intercellular adhesion
molecule-1 (Icam-1) to proximal mouse chromosome 9. Genomics 9: 547-550, 1991.
2. Bella, J.; Kolatkar, P. R.; Marlor, C. W.; Greve, J. M.; Rossmann, M. G. The structure of the two amino-terminal
domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. Proc.
Nat. Acad. Sci. 95: 4140-4145, 1998.
3. Simmons, D.; Makgoba, M. W.; Seed, B. ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell
adhesion molecule NCAM. Nature 331: 624-627, 1988.
SpecificityNatural and recombinant mouse sICAM-1
ImmunogenExpression system for standard: NS0; Immunogen sequence: Q28-N485
DescriptionFor quantitative detection of mouse ICAM-1 in cell culture supernatants, serum and plasma(heparin, EDTA).
Gene SymbolICAM1
Gene Full Nameintercellular adhesion molecule 1
Alias SymbolsBB2, CD 54, CD54, CD54 antigen, Cell surface glycoprotein P3.58, Human rhinovirus receptor, ICAM 1, Intercellular adhesion molecule 1, Major group rhinovirus receptor, MALA2, MGC6195, MyD10, P3.58, Surface antigen of activated B cells
NCBI Gene Id15894
Protein NameIntercellular adhesion molecule 1
Description of TargetIntercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE.
Uniprot IDP13597
Protein Accession #NP_034623.1
Nucleotide Accession #NM_010493.2
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