IBSP ELISA Kit (Mouse) (OKEH04444)

Catalog No: OKEH04444

Size:96T

Mouse IBSP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ibsp ELISA Kit (Mouse) (OKEH04444) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ibsp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Ibsp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ibsp captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.3% (n = 20)
  Mean Inter-assay CV%: <= 7.7% (n = 20)
• ELISA Kit Component:
  • Anti-Ibsp Microplate: 96 Wells (12 x 8 Well Strips)
  • Ibsp Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Ibsp Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.163 ng/mL
• Specificity: Natural and recombinant Mouse Bone sialoprotein 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ibsp
• Gene Full Name: integrin binding sialoprotein
• Alias Symbols: B;bone sialoprotein 2;bone sialoprotein II;Bs;BSP;BSP II;Bsp2;BSPII;cell-binding sialoprotein;Integrin-binding sialoprotein.
• NCBI Gene Id: 15891
• Protein Name: Q61711
• Description of Target: Bone sialoprotein 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q61711
• Protein Accession #: NP_032344.2
• Nucleotide Accession #: NM_008318.3
• Protein Size (# AA): 324
• Molecular Weight: 36 KDa