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Datasheets/Manuals | Printable datasheet for human FGF2 Polyclonal Antibody (OABB00823) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | Enzyme-linked immunosorbent assay|Flow cytometry-intracellular|Immunocytochemistry|Immunofluorescence|Western blot |
Additional Information | Notes: By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor(FGF) family. Fibroblast growth factors(FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | E.coli-derived human FGF2 recombinant protein (Position: P143-S288). |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Dilution | Optimal dilutions should be determined by end user. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat ELISA : 0.1-0.5 ug/ml: Human |
Reference | 1. Doniach, T. : Basic FGF as an inducer of anteroposterior neural pattern. Cell 83: 1067-1070, 1995 2. Eckenstein, F. P. : Fibroblast growth factors in the nervous system. J. Neurobiol. 25: 1467-1480, 1994. |
Storage Buffer | Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Fibroblast growth factor 2(FGF2) detection. Tested with WB, IHC-P, ELISA in Human. |
Gene Symbol | FGF2 |
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Gene Full Name | fibroblast growth factor 2 |
Alias Symbols | Basic fibroblast growth factor;basic fibroblast growth factor bFGF;BFGF;FGF-2;FGFB;fibroblast growth factor 2;fibroblast growth factor 2 (basic);HBGF-2;heparin-binding growth factor 2;prostatropin. |
NCBI Gene Id | 2247 |
Protein Name | Fibroblast growth factor 2 |
Description of Target | Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). |
Uniprot ID | P09038 |
Molecular Weight | 30770 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "human FGF2 Polyclonal Antibody (OABB00823)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "human FGF2 Polyclonal Antibody (OABB00823)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "human FGF2 Polyclonal Antibody (OABB00823)" provided in?
This item is provided in "Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "human FGF2 Polyclonal Antibody (OABB00823)"?
This target may also be called "Basic fibroblast growth factor;basic fibroblast growth factor bFGF;BFGF;FGF-2;FGFB;fibroblast growth factor 2;fibroblast growth factor 2 (basic);HBGF-2;heparin-binding growth factor 2;prostatropin." in publications.
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What is the shipping cost for "human FGF2 Polyclonal Antibody (OABB00823)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "human FGF2 Polyclonal Antibody (OABB00823)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "human FGF2 Polyclonal Antibody (OABB00823)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "30770 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "human FGF2 Polyclonal Antibody (OABB00823)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "FGF2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "FGF2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "FGF2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "FGF2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "FGF2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "FGF2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.