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Catalog No: OKEH00612
Size:96T
Price: $525.00
SKU
OKEH00612
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HSPD1 ELISA Kit (Mouse) : 96 Wells (OKEH00612)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HSPD1 ELISA Kit (Mouse) (OKEH00612) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPD1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-HSPD1 Microplate96 Wells (12 x 8 Well strips)
HSPD1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HSPD1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse 60 kDa heat shock protein, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHspd1
Gene Full Nameheat shock protein 1 (chaperonin)
Alias Symbols60 kDa chaperonin;60 kDa heat shock protein, mitochondrial;60kDa;chaperonin 60;CPN60;heat shock 60kDa protein 1 (chaperonin);heat shock protein 60;heat shock protein, 60 kDa;Hsp;Hsp60;HSP-60;HSP-65;mitochondrial matrix protein P1.
NCBI Gene Id15510
Protein NameP63038
Description of Target60 kDa heat shock protein, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P63038
Protein Accession #NP_034607.3
Nucleotide Accession #NM_010477.4
Protein Size (# AA)537
Molecular Weight60 kDa
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