Catalog No: OKEH01108
Size:96T
Price: $525.00
SKU
OKEH01108
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HSPA5 ELISA Kit (Rat) : 96 Wells (OKEH01108)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HSPA5 ELISA Kit (Rat) (OKEH01108) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPA5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA5 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
HSPA5 Microplate96 Wells (12 x 8 Well strips)
HSPA5 Lyophilized Standard2
100X Biotinylated HSPA5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.17 ng/mL
SpecificityNatural and recombinant Rat 78 kDa glucose-regulated protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHspa5
Gene Full Nameheat shock protein family A (Hsp70) member 5
Alias Symbols78 kDa glucose-regulated protein;binding-immunoglobulin protein;BIP;endoplasmic reticulum chaperone BiP;GRP 78;GRP78;GRP-78;heat shock 70 kDa protein 5;heat shock 70kD protein 5;heat shock 70kDa protein 5 (glucose-regulated protein);heat shock protein 5;heat shock protein 70 family protein 5;heat shock protein family A member 5;HSP70 family protein 5;immunoglobulin heavy chain-binding protein;steroidogenesis-activator polypeptide.
NCBI Gene Id25617
Protein NameP06761
Description of TargetEndoplasmic reticulum chaperone BiP
Uniprot IDhttps://www.uniprot.org/uniprot/P06761
Protein Accession #NP_037215.1
Nucleotide Accession #NM_013083.2
Protein Size (# AA)654
Molecular Weight72 kDa
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