HSPA12B ELISA Kit (Human) (OKCD01084)

Catalog No: OKCD01084 (Formerly GWB-KBBZL8)

Size:96WELLS

Human HSPA12B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 12B (HSPA12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 12B (HSPA12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 12B (HSPA12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock 70kDa Protein 12B (HSPA12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 12B (HSPA12B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 12B (HSPA12B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HSPA12B Microplate: 96 Wells (12 x 8 Well Strips)
  • HSPA12B Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated HSPA12B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.128 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 12B (HSPA12B). No significant cross-reactivity or interference between Heat Shock 70kDa Protein 12B (HSPA12B) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HSPA12B
• Gene Full Name: heat shock protein family A (Hsp70) member 12B
• Alias Symbols: C20orf60;heat shock 70 kDa protein 12B.
• NCBI Gene Id: 116835
• Protein Name: Heat shock 70 kDa protein 12B
• Description of Target: Heat shock 70 kDa protein 12B
• Uniprot ID: Q96MM6
• Protein Accession #: NP_001184256
• Nucleotide Accession #: NM_001197327.1
• Protein Size (# AA): 686