Catalog No: OKEH03225
Size:96T
Price: $525.00
SKU
OKEH03225
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HSP90B1 ELISA Kit (Mouse) (OKEH03225) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsp90b1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hsp90b1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsp90b1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.9% (n = 20)
Mean Inter-assay CV%: <= 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Hsp90b1 Microplate96 Wells (12 x 8 Well strips)
Hsp90b1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Hsp90b1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.081 ng/mL
SpecificityNatural and recombinant Mouse Endoplasmin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHsp90b1
Gene Full Nameheat shock protein 90, beta (Grp94), member 1
Alias Symbols94 kDa glucose-regulated protein;end;endoplasmic reticulum resident protein 99;endoplasmin;ERp;ERp99;gp9;gp96;GRP;GRP94;heat shock protein 90 kDa beta member 1;polymorphic tumor rejection antigen 1;TA-3;Tar;Targ2;Tr;Tra;Tra1;Tra-1;transforming growth factor alpha regulated gene 2;tumor rejection antigen (gp96) 1;tumor rejection antigen gp96.
NCBI Gene Id22027
Protein NameP08113
Description of TargetEndoplasmin
Uniprot IDhttps://www.uniprot.org/uniprot/P08113
Protein Accession #NP_035761.1
Nucleotide Accession #NM_011631.1
Protein Size (# AA)802
Molecular Weight92 kDa
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