HSP90B1 ELISA Kit (Human) (OKEH00613)

Catalog No: OKEH00613

Size:96T

Human HSP90B1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HSP90B1 ELISA Kit (Human) (OKEH00613) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSP90B1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSP90B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSP90B1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.5% (n = 20)
• ELISA Kit Component:
  • HSP90B1 Microplate: 96 Wells (12 x 8 Well strips)
  • HSP90B1 Lyophilized Standard: 2
  • 100X Biotinylated HSP90B1 Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.041 ng/mL
• Specificity: Natural and recombinant Human Endoplasmin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HSP90B1
• Gene Full Name: heat shock protein 90 beta family member 1
• Alias Symbols: 94 kDa glucose-regulated protein;ECGP;endoplasmin;endothelial cell (HBMEC) glycoprotein;epididymis luminal protein 35;epididymis secretory sperm binding protein Li 125m;GP96;gp96 homolog;GRP94;heat shock protein 90 kDa beta member 1;heat shock protein 90kDa beta (Grp94), member 1;heat shock protein 90kDa beta family member 1;HEL35;HEL-S-125m;stress-inducible tumor rejection antigen gp96;TRA1;tumor rejection antigen (gp96) 1;tumor rejection antigen 1.
• NCBI Gene Id: 7184
• Protein Name: P14625
• Description of Target: Endoplasmin
• Uniprot ID: https://www.uniprot.org/uniprot/P14625
• Protein Accession #: NP_003290.1
• Nucleotide Accession #: NM_003299.2
• Protein Size (# AA): 803
• Molecular Weight: 92 kDa