HSD17B14 ELISA Kit (Human) (OKCD00457)

Catalog No: OKCD00457 (Formerly GWB-KBBTT7)

Size:96WELLS

Human HSD17B14 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HSD17B14 Microplate: 96 Wells (12 x 8 Well Strips)
  • HSD17B14 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated HSD17B14 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HSD17B14
• Gene Full Name: hydroxysteroid 17-beta dehydrogenase 14
• Alias Symbols: 17-beta-HSD 14;17-beta-hydroxysteroid dehydrogenase 14;17-beta-hydroxysteroid dehydrogenase DHRS10;dehydrogenase/reductase (SDR family) member 10;Dehydrogenase/reductase SDR family member 10;DHRS10;retinal short-chain dehydrogenase/reductase 3;retinal short-chain dehydrogenase/reductase retSDR3;retSDR3;SDR47C1;short chain dehydrogenase/reductase family 47C member 1;testicular tissue protein Li 52.
• NCBI Gene Id: 51171
• Protein Name: 17-beta-hydroxysteroid dehydrogenase 14
• Description of Target: Has NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity. Converts oestradiol to oestrone. The physiological substrate is not known. Acts on oestradiol and 5-androstene-3-beta,17-beta-diol (in vitro).
• Uniprot ID: Q9BPX1
• Protein Accession #: NP_057330
• Nucleotide Accession #: NM_016246.2
• Protein Size (# AA): 270